DNP (2,4-Dinitrophenol, 2,4-DNP)

Size

£832.00

SKU:: 052715
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: 2,4-Dinitrophenol
Format:: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, 50% glycerol.
Extra Details:: 2,4-Dinitrophenol (DNP) is a hapten that induces a strong immune response when attached to proteins or peptides. DNP can be incorporated at protein carbonyls via covalent linkage of DNP to carbonyl groups using 2,4-dinitrophenylhydrazine (DNPH) or through site-specific incorporation of unnatural amino acids that contain a DNP functional group. Immunization with DNP-modified proteins or tumor cells induces antibody formation in vivo and has been used in the design of vaccines for clearance of disease-causing cells or viruses and induction of autoimmune diseases such as colitis and thyroiditis in various animal models. Applications: Suitable for use in Immunoprecipitation, ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:1000 Western Blot: 1:1000 Immunoprecipitation: 50ul/test Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.