Ricin A Chain

Size

£780.00

SKU:: 052675
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified Ricin A chain.
Clone:: RAC18
Format:: Supplied as a liquid in PBS. No preservative added.
Extra Details:: Ricin is a dimeric toxin derived from Ricinus communis (Caster bean). It is composed of a sugar-binding subunit (B chain) that attached to cell surface receptors and facilitate the entry of toxin into the target cell and a second subunit (A chain), whcih has glycosidase activity that inactivates ribosomes by depurinating a single adenosine residue from an exposed loop of the 28S ribosomal RNA. This leads to inhibition of protein synthesis in cells. The toxic effects of Ricin are attributed to the A chain. 052675 displays high avidity for intact ricin and inhibits its activity without affecting binding of ricin to cells. Immunization is shown to offer significant protection against ricin toxicity. Applications: Suitable for use in Indirect ELISA, Inhibition assays and Neutralization. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.