Transforming Growth Factor alpha (TGFa)

Size

£971.00

SKU:: 052668
Purity:: Purified by immunoaffinity chromatography. Endotoxin: ≤0.1EU/ug (LAL)
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Recombinant protein corresponding to Val40-Ala89 from human TGF alpha, expressed in E. coli.
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: TGF-alpha was originally isolated from the conditioned media of oncogenically transformed cells as an EGF-like bioactivity. TGF-alpha is a member of the EGF family of cytokines that are synthesized as transmembrane precursors and are characterized by the presence of one or several EGF structural units in their extracellular domain. The soluble forms of these cytokines are released from the transmembrane protein by proteolytic cleavage. Membrane-bound proTGF-alpha is biologically active and seems to play a role in mediation of cell-cell adhesion and in juxtacrine stimulation of adjacent cells. Expression of TGF-alpha is widespread in tumors and transformed cells. TGF-alpha is also expressed in normal tissues during embryogenesis and in adult tissues, including pituitary, brain, keratinocytes and macrophages. Mature TGF-alpha shows approximately 93% amino acid sequence identity with mouse or rat TGF-alpha and is not species specific in its biological effects. TGF-alpha binds to the EGF receptor and activates the receptor tyrosine kinase. Accordingly, TGF-alpha shows a similar potency to EGF as a mitogen for fibroblasts and as an inducer of epithelial development in vivo. TGF-alpha is reportedly more potent than EGF as an angiogenic factor in vivo and as a stimulator for keratinocyte migration. The EGF receptor gene represents the cellular homologue of the avian v-erb-B oncogene. Applications: Suitable for use in Sandwich ELISA, Immunohistochemistry, Neutralization and Western Blot. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.1ug/ml. Immunohistochemistry: 5-15ug/ml using immersion fixed paraffin-embedded sections of human astrocytoma. Neutralization: 0.15-0.75ug/ml in the presence of 3ng/ml recombinant human TGF-a. Measured by its ability to neutralize TGF‑ alpha -induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. ELISA (Sandwich): 0.2-0.8ug/ml. Optimal dilutions to be determined by researcher. Matched Pair: Capture: 052668 Detection: 052667 Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice