Green Fluorescent Protein (GFP)

Size

£544.00

SKU:: 052654
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Chicken/Avian
Immunogen:: Recombinant protein corresponding to GFP expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, 50% glycerol.
Extra Details:: Green Fluorescent Protein (GFP) is a naturally fluorescent protein originally derived from jellyfish. GFP has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors which have become useful and ubiquitous tools in transgenic experiments. Fluorescent proteins enable a wide range of applications where they have functioned as cell lineage tracers, reporters of gene expression, or as a measure of protein-protein interactions. Applications: Suitable for use in ELISA, Immunocytochemistry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:5000-1:10,000 Immunohistochemistry: 1:2000-1:5000 Immunocytochemistry: 1:2000-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.