PTHrP (Parathyroid Hormone-related Protein, PTHrP, PTH-rP, PTH-Related Protein, Parathyroid Hormone-like Protein, PLP)

Size

£767.00

SKU:: 052649
Application:: WB
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: GST-tagged recombinant protein corresponding to human PTHrP lacking signal and propeptide sequence.
Clone:: 4C4.1
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Extra Details:: Parathyroid hormone-related protein (UniProt P12272; also known as Parathyroid hormone-like related protein, Parathyroid hormone-like hormone preproprotein, PLP, PTH-related protein, PTH-rP, PTHrP) is encoded by the PTHLH (also known as BDE2, HHM, PTHR, PTHRP) gene (Entrez Gene ID 5744) in human. Parathyroid hormone-related protein is initially translated as a 177 amino acid preproprotein with an N-terminal signal sequence (aa 1-24) and a propeptide sequence (aa 25-34), which are later removed to yield the mature protein. The mature protein is further cleaved to produce PTHrP[1-34] (aa 37-70), PTHrP[1-36] (aa 37-72), PTHrP[38-94] (aa 74-130), and PTHrP[107-139] (aa 143-175; also known as Osteostatin). PTHrP is produced in a wide variety of tissues, where it acts as a paracrine or autocrine factor to regulate cellular and organ growth, development, migration, differentiation, calcium transport, survival and apoptosis. Applications: Suitable for use in Western Blot. Other applications have not been tested. Recommended Dilutions: Western Blot: 2ug/ml detected PTHrP in 10ug of MCF-7 cell lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice