Glutathione S-Transferase, Epitope Tag (GST-Tag)

Size

£639.00

SKU:: 052632
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Glutathione-S-Transferase (GST) tagged protein
Clone:: 3E2
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 50% glycerol.
Extra Details:: Glutathione S-transferases (GSTs) are a family of detoxifying enzymes that play a crucial role in cellular defense against oxidative stress. By catalyzing the conjugation of glutathione to a wide range of electrophilic compounds, GSTs help neutralize reactive oxygen species (ROS) and prevent cellular damage. In the context of neurodegenerative diseases, GSTs are increasingly recognized for their neuroprotective functions. Oxidative stress is a key pathological feature in disorders such as Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis (ALS). GSTs mitigate this stress by detoxifying lipid peroxidation products and other neurotoxic metabolites, thereby preserving neuronal integrity. Several GST isoforms are expressed in the brain, and their expression levels are often altered in neurodegenerative conditions. Polymorphisms in GST genes have also been linked to increased susceptibility to neurodegeneration, suggesting a genetic component to their protective role. Beyond detoxification, GSTs may influence cell signaling pathways involved in inflammation, apoptosis, and mitochondrial function—processes that are dysregulated in neurodegeneration. As such, GSTs are being explored as potential biomarkers and therapeutic targets for early intervention and disease modification in neurodegenerative disorders. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 for detection of GST in 1ug of purified GST tagged PmtA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. ELISA: 1:800 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice