VEGFR1 (Vascular Endothelial Growth Factor Receptor 1, Flt-1)

Size

£902.00

SKU:: 052630
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Ser27-His687 from human VEGFR1, expressed in Sf21 cells.
Clone:: 25H11
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) is a receptor tyrosine kinase that is expressed primarily on endothelial cells and plays a role in vasculogenesis and angiogenesis. A soluble variant of VEGFR1 was also reported to bind VEGF and PlGF with high affinity and function as a potent VEGF antagonist. Applications: Suitable for use in Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using recombinant human VEGFR1/Flt-1 Fc Chimera under non-reducing conditions only. Flow Cytometry: 2.5ug/10e6 cells in HUVEC human umbilical vein endothelial cells. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.