MDM2

Size

£800.00

SKU:: 052625
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: His-tagged recombinant protein corresponding to human MDM2. Species Sequence Homology: mouse (100%)
Clone:: 3G9
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Extra Details:: MDM2 is over-expressed in many tumors. Its principal function is the ubiquitination and degradation of p53 tumor suppressor protein. This monoclonal antibody also recognizes a peptide epitope around Thr-216 of murine MDM2 when Thr-216 is unphosphorylated. Applications: Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml detected MDM2 on 10ug of MCF-7 cell lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.