Eosinophil Peroxidase (EPO, EPX)

Size

£672.00

SKU:: 052610
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human Eosinophils from a patient with hypereosinophilic syndrome.
Clone:: AHE-1
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Extra Details:: Eosinophil peroxidase (UniProt: P11678; also known as EC:1.11.1.7, EPO, EPX) is encoded by the EPX (also known as EPER, EPO, EPP) gene (Gene ID: 8288) in human. Eosinophil peroxidase is an enzyme found in cytoplasmic granules of eosinophils. It mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. It is synthesized with a signal peptide (aa1-17) and a propeptide (aa18-139), which are subsequently cleaved off to produce the mature form that can be cleaved into a light chain (aa140-250) and a heavy chain (aa251-715). These chains can assemble to for a tetrameric structure. It is a heme-containing enzyme that physically associates with fibrous tissue and cancer tissue in various organs. It contains well-conserved profibrogenic capacity to stimulate the migration of fibroblastic cells and promote their ability to secrete collagenous proteins to generate a functional extracellular matrix (ECM) both in vitro and in vivo. It plays a role in regulating the recruitment of fibroblast and the biosynthesis of collagen ECM at sites of normal tissue repair and fibrosis. It is a key participant in generation of reactive oxidants and diffusible radical species by the phagocytes. It serves as a potent toxin towards invading parasites and the surrounding tissues. It also displays significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial fragmentation and lysis. Applications: Suitable for use in Immunocytochemistry, Immunofluorescence and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunofluorescence: 1:50 detected Eosinophil Peroxidase in frozen human colon tissue sections. Immunohistochemistry: formalin-fixed, paraffin-embedded sections Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice