Milk Fat Globule-EGF Factor 8, Bovine

Size

£889.00

SKU:: 052600
Molecular Weight:: 52000
Purity:: ≥95% (SDS-PAGE)
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 70mM sodium phosphate, pH 7.0. No preservative added.
Extra Details:: Lactadherin is a widely distributed glycoprotein (~ 50kD), which was originally characterized due to its association with milk fat/lipid globule membranes. Synonymous names are PAS-6/7, bovine-associated mucoprotein, BA-46, P47, and MFG-E8. Structural hallmarks of lactadherin are the presence of two epidermal growth factor (EGF) homology domains (with an RGD peptide motif in the second EGF domain), and two C domains sharing homology with the discoidin family of lectin domains including the phospholipid-binding domains of blood clotting factors V and VIII. Lactadherin shows preferential binding to phosphatidylserine (L-form) in a calcium independent manner. Purified lactadherin functions as an anticoagulant by blocking phosphatidylserine-containing membrane sites for blood coagulation proteins (10). Fluoresence-labeled lactadherin functions as a sensitive probe for exposed phosphatidylserine on nucleated cells and on stimulated platelets (8, 9). Lactadherin will bind to membranes that have phosphatidylserine content below the threshold for annexin V binding. Localization: Milk (1), also secreted by stimulated macrophages (2), vascular smooth muscle (3), endothelial cells(4) Plasma Concentration: Unknown in normal adults, measurable in women with metastatic breast cancer (5), measurable in newborn calves after milk meal (1). Molecular Weight: 52kD and 47kD (glycosylation variants, PAS-6 and PAS-7 respectively) Extinction Coefficient: E1%, 1cm, 280nm = 16.5 Structure: Single chain with two EGF domains and two C domains. The mouse isoform contains a central proline rich region and the human molecule contains a single EGF domain Percent Carbohydrate: 6%, 13% for glycosylation variants Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Dry Ice