052594
Glutathione: N-ethylmaleimide Adduct (GS-NEM)
Size
£767.00
- SKU:
- 052594
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- GS-NEM conjugated to KLH. Species Sequence Homology: mammals
- Clone:
- 8.1GSH
- Format:
- Supplied as a liquid in PBS, pH 7.6, 0.09% sodium azide.
- Extra Details:
- 052594 is specific to the glutathione:N-ethylmaleimide adduct; thus it is useful for quantification of reduced glutathione (GSH) after reaction with N-ethylmaleimide. For use in ELISA or flow Cytometry, sample must first be reacted with NEM. Applications: Suitable for use in Western Blot, ELISA, Flow Cytometry, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (paraformaldehyde fixation): 1:100. Immunocytochemistry: 1:100-1:500 for assessment of intracellular GSH distribution. ELISA: 1:100,000-1:200,000 to quantify GSH at >2mM in biological specimens. Flow Cytometry: 1:100-1:1000. Cells were fixed with cold 1% PFA for 5 min and vortexed into methanol (-20°C) containing 5mM NEM (10 min on dry ice) or 10uM NEM (N-ethyl maleimide) for 30 min at RT. Optimal dilutions to be determined by the researcher. Positive Control: Peripheral blood mononuclear cells (PBMC) treated with NEM Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice