LIG1 (DNA Ligase 1)

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£453.00

SKU:: 052590
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to human DNA Ligase I.
Clone:: 7B2 (recomb)
Format:: Supplied as a liquid in PBS, pH 7.4, 0.05% BSA, 0.02% sodium azide, 50% glycerol.
Extra Details:: LIG1 is the main replicative ligase involved in DNA replication, recombination, and the base excision repair process. LIG1 is associated with the replisome to finalize Okazaki fragment maturation, completing more than 50 million ligation events during each cycle of DNA replication. It also ligates single-strand DNA breaks during long-patch base excision repair (BER) and can participate in the alternative end-joining (A-EJ) pathway. LIG1 employs a unique metal-mediated fidelity mechanism whereby rigidity imposed by metal binding allows for discrimination against base mismatches on the 3′-hydroxyl side of the nick. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:5000. Immunohistochemistry (paraffin): 1:50-1:200. Requires HIER. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice