Nucleoporin p62, NT (NUP62)

Size

£620.00

SKU:: 052578
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein containing amino acid residues in the N-terminal region of human nucleoporin p62
Clone:: M436
Format:: Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide, 50% glycerol.
Extra Details:: Active transport of proteins and RNA into and out of the nucleus occurs via the nuclear pore complex (NPC). The NPC is formed by a multiprotein complex that includes nucleoporin proteins. Specific nuclear localization sequences found in proteins target proteins for active transport into the nucleus through the NPC. Nucleoporin p62 is the best characterized member of the family of nucleoporins found in the NPC. A tightly associated complex is formed by p62 and two other nucleoporins, p54 and p58. p54 binds to a carboxy-terminal coiled-coil domain of p62 and p58 binds to a dimer of p54. The amino-terminal domain of p62 contains a series of XFXFX repeats and is joined to the coiled-coil domain by a threonine-rich linker segment. The major role of p62 is maintenance of the structural integrity of NPCs. Applications: Suitable for use in Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:500. Immunocytochemistry: 1:50, specifically stains nuclei and nuclear envelope in paraformaldehyde fixed and NP-40 permeabilized cells. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice