Lymphotoxin beta Receptor (Lymphotoxin B Receptor, LTbetaR, LT-beta-R, LTBR, AI256028, CD18, Ltar, TNF-R-III, Tnfbr, TNFRrp, TNFR-RP, TNFR2-RP, Tumor Necrosis Factor C Receptor, TNFCR, Tumor Necrosis Factor Receptor Superfamily Member 3, Tnfrsf3) (Low Endotoxin)

Size

£1,190.00

SKU:: 052562
Application:: Flow Cytometry
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to the extracellular domain of mouse LT beta R
Clone:: 5G11b
Format:: Supplied as a liquid in PBS. No preservative added.
Extra Details:: Lymphotoxin beta receptor is 385 amino acid (415 aa including 30 aa signal peptide) ~50kD type 1, single pass transmembrane glycoprotein and member of the tumor necrosis factor family. LT beta R is primarily expressed on stromal cells and fibroblasts and at lower levels on myeloid cell lines. 052562 is reported to be agonistic with respect to NF Kappa B activation, MIP-2 induction and IL-6 release (Hehlgans et al. 2003). 052562 is routinely tested on WEH164S cells. Applications: Suitable for use in Flow Cytometry and Functional Assays. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: SP2/0-Ag14 myeloma cells with spleen cells from Wistar rats. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.