052542
Active Caspase-3
Size
£364.00
- SKU:
- 052542
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human Active Caspase-3. Cellular Localization: Cytoplasm
- Clone:
- 30C11
- Format:
- Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Active caspase-3 is a protein encoded by the CASP3 gene which is approximately 31,6kD. Active caspase-3 is localised to the cytoplasm. It is involved in the TNFR1 pathway, apoptosis modulation and signalling and respiratory electron transport. This protein falls under the cysteine-aspartic acid protease family. It is a sequential activator of caspases and plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Active caspase-3 is highly expressed in lung, spleen, heart, liver and kidney. Mutations in the CASP3 gene may result in bladder urothelial carcinoma and primary effusion lymphoma. Applications: Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1:500-1:5000 Immunohistochemistry (Paraffin): 1:500-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice