Synaptopodin (SYNPO, KIAA1029)

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£473.00

SKU:: 052507
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Isolated rat kidney glomeruli.
Clone:: 5F198
Format:: Supplied as a liquid in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. For antibody in lyophilized powder format see S9500-74.
Extra Details:: Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin, represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7kD and pI of 9.38 (calculated from sequence data); in mouse the corresponding data are 74kD, pI 9.27. In SDS-PAGE the antigen appears as 100kD polypeptide in brain and 110kD polypeptide in kidney (the difference might be attributed to posttranslational modifications). Applications: Suitable for use in Immunofluorescence/Immunocytochemistry, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Immunohistochemistry (Frozen): 1:50-1:200 (0.25-1ug/ml) Immunohistochemistry (paraffin): 1:50-1:200 (0.25-1ug/ml) Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice