TKTL1 (Transketolase-Like 1)

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£646.00

SKU:: 052502
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: HPLC purified peptide from TKTL1
Clone:: 7H2-G5-C1
Format:: Supplied as a lyophilized powder in PBS, pH 7.4, 0.5% BSA. Reconstitute with 1ml sterile ddH2O.
Extra Details:: Expression studies on transketolase-like-1 show that the protein is found in aggressive tumors. The detection of TKTL1 is furthermore correlated with poor patient prognosis and metastasis. Transketolase dependent reactions regulate the non-oxidative part of the pentose-phosphate-pathway (PPP) in glucose metabolism. This pathway generates NADPH and pentose phosphates. The latter are important for nucleic acid metabolism. Beside the ubiquitous transketolase (TKT) other transketolase-like proteins exist. Transketolase-like 1 (TKTL1) is a protein with a 38aa deletion when compared to TKT. TKTL1 expression has been shown in tumors of different entities. Inhibition of TKTL1 expression by RNA interference results in reduced growth of tumor cells, while overexpression of TKTL1 promotes cell proliferation. Recent publications suggest an important role of TKTL1 in the metabolism of tumor cells. In colorectal tumors nuclear as well as cytoplasmic localization has been described. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 1:50 (perform heat-induced epitope retrieval by heating the paraffin sections at 95 °C for 30 min in 10mM citrate buffer (pH 6.0) Western Blot: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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