CD45 (PE)

Size

£1,075.00

SKU:: 052492
Application:: Flow Cytometry
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Glycoproteins isolated from the T cell line, RL-5.
Clone:: L12/201
Format:: Supplied as a lyophilized powder from PBS, pH 7.2, 1% BSA, 5% sucrose, 0.09% sodium azide. Labeled with R.-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.
Extra Details:: Immunoprecipitation was only obtained after cross linking antibody to the labelled cell surface. This gave a band of ~200kD. Applications: Suitable for use in Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: Neat 1:10; 10ul labels 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher. Hybridoma: P3.X63.Ag8-U1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months after receipt at 4°C. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.