Siglec 1 (Sialic Acid Binding Ig-like Lectin 1, Sialoadhesin, CD169)

Size

£825.00

SKU:: 052475
Purity:: Purified by Protein G affinity chromotography from hybridoma culture supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to Thr20-Leu1639 (predicted) of mouse Siglec-1, expressed in NS0 cells
Clone:: 25E1
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Siglec-1, also known as sialoadhesin or CD169, is a 175‑185kD type I transmembrane glycoprotein belonging to the Siglec family of sialic acid specific I-type lectins within the immunoglobulin superfamily. Mouse Siglec-1 contains a 1619 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set domain and 16 Ig-like C2-set domains. The ECD shares 73% and 83% aa sequence identity with human and rat Siglec-1, respectively. Alternate splicing generates two soluble isoforms containing either 16 or the first 3 Ig-like domains. Siglec-1 is expressed by some tissue macrophages, dendritic cells and circulating monocytes during certain infections. It binds sialylated molecules including MMR, MGL1/CD301a, MUC1, PSGL-1 and CD43. Applications: Suitable for use in Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using lysates of mouse splenocytes Flow Cytometry: 0.25ug/10e6 cells with RAW 264.7 mouse monocyte/macrophage cells stimulated with 1ug/ml LPS overnight Optimal dilutions to be determined by the researcher. Storage and Stablility: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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