Annexin A5 (ANXA5)

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£576.00

SKU:: 052464
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Full length recombinant protein corresponding to human annexin A5 expressed in E. coli.
Clone:: 6A12
Format:: Supplied as a liquid in PBS, 5mM sodium azide, 50% glycerol
Extra Details:: The annexins are a large family of related proteins which share the property of binding to phophospholipid containing membranes in a Calcium dependent manner (1). Different members of the family were discovered by different laboratories and as a result the various members have many alternate names, such as lipocortin, calpactin, calelectrin and others. In fact Annexin A5 has a particularly surprising number of alternate names, 20 being listed on the Genecards ANXA5 site. The widely used current nomenclature is now based on a letter to indicate membership in a particular one of several annexin sub-families and a number for individual gene products, hence the name annexin A5. The annexin family is defined by a compact disc structure formed from 16 closely packed α-helices which coordinate multiple calcium ions with phospholipid containing membranes. This domain is defined by 4 imperfect repeats of a ~77aa sequence, each repeat forming 4 α-helices (2,3). Annexin A5 is expressed widely in tissues and has been used as a marker of apoptosis, as apoptotic cells may express binding sites for this protein on their cell surface. The protein binds to phosphatidylserine, a membrane lipid normally not found on the external surface of cells which becomes expressed on the cell surface during apoptosis. As a result fluorescent annexin A5 or annexin A5 antibody can be used to isolate apoptotic cells by fluorescence activated cell sorting (4). Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.Immunofluorescence. Other applications not tested. Recommended Dilutions: Western Blot: 1:2000-1:5000 Immunohistochemistry: 1:1000-1:2000 Immunofluorescence (IC): 1:1000-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice