052461
PBEF (Pre-B cell Colony Enhancing Factor, Pre-B cell Enhancing Factor, Nicotinamide Phosphoribosyltransferase, NAmPRTase, NAMPT, Pre-B cell Colony Enhancing Factor 1, PBEF1, Visfatin)
Size
£856.00
- SKU:
- 052461
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- E. coli-derived recombinant protein corresponding to Pro27-His491 from human PBEF/Visfatin.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- PBEF (pre-B cell colony-enhancing factor; also visfatin and nicotinamide phosphoribosyltransferase) is a 52kD member of the NAPRTase family of molecules. It functions both intracellularly and extracellularly, where it participates in NAD synthesis and insulin receptor activation, respectively. Human PBEF is 491 amino acids in length and contains no signal sequence. There is at least one alternative splice form that shows a 5 aa substitution for the C-terminal 128 amino acids (aa 364-491). Over aa 27-491, human PBEF shares 96%, 97% and 96% aa identity with mouse, porcine and canine PBEF, respectively. Applications: Suitable for use in Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot: 0.2ug/ml with lysates of human heart tissue, 293T human embryonic kidney cell line, and A431 human epithelial carcinoma cell line under reducing conditions. Flow Cytometry (Intracellular): 0.25ug/10e6 cells. Human peripheral blood mononuclear cells were treated for 24 hours with 50ng/ml PMA stained with 052461 and cells were fixed with paraformaldehyde and permeabilized with saponin. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice