052458
PPAD (Porphyromonas gingivalis)
Size
£731.00
- SKU:
- 052458
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to P. gingivalis PPAD
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Porphyromonas gingivalis (P. gingivalis) peptidyl arginine deiminase (PPAD) is a bacterial enzyme and virulence factor secreted by P. gingivalis, an oral bacterium involved in the progression of periodontal disease. It is composed of a pro-peptide sequence, a catalytic domain, an immunoglobulin-like (Ig-like) domain, and a C-terminal domain, but the mature protein contains only the catalytic domain and Ig-like domain. It is expressed on the bacterial outer membrane. PPAD and proteolytic enzymes, such as arginine gingipain (RgpB), are released from P. gingivalis to break down proteins, which it uses as an energy source. RgpB cleaves proteins at arginine residues, and PPAD specifically deiminates the resulting C-terminal arginine residues of both bacterial and host proteins to citrulline, a process known as citrullination. Unlike mammalian PADs, PPAD does not require calcium and can convert free L-arginine into L-citrulline in addition to deaminating these residues at the C-terminus of proteins and peptides. A ppad deletion mutation (Δ8820) in P. gingivalis strain 381 increases biofilm formation, alters biofilm architecture, and induces the secretion of protein aggregates. Mice infected with a PPAD deficient strain of P. gingivalis have reduced periodontal inflammation and similarly infected mice in a model of collagen-induced arthritis have reduced bone erosion. Applications: Suitable for use in ELISA, Flow Cytometry, Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:200 Immunofluorescence: 5ug/ml Optimal dilutions to be determined by the researcher.her. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice