052451
Rho (-A, -B, -C)
Size
£698.00
- SKU:
- 052451
- Application:
- WB
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- KLH-conjugated synthetic peptide corresponding to amino acids 73-94 (SYPDTDVILMCFSIDSPDSLEN-KK) of human RhoA and RhoB, with two C-terminal lysines added to increase peptide solubility corresponding to human Rho (-A, -B, -C). Species Sequence Homology: chicken, rat, zebrafish, mouse, nematode and Xenopus
- Format:
- Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
- Extra Details:
- Rac1, RhoA, and Cdc42 (UniProt P63000, P61586, and P60953, respectively) are among the most studied Rho family of small GTPases that are involved in a wide variety of cellular functions such as cell polarity, vesicular trafficking, and cell cycle by regulating intracellular actin dynamics. In mammals, the Rho family contains 22 members which can be further divided into the Rac subfamily (Rac1, Rac2, Rac3, and RhoG), Cdc42 subfamily (Cdc42, TC10, TCL, Chip, and Wrch-1), RhoA subfamlly (RhoA, RhoB, and RhoC) and other Rho-GTPases (RhoE/Rnd3, RhoH/TTF, Rif, RhoBTB1, RhoBTB2, Miro-1, Miro-2, RhoD, Rnd1, and Rnd2). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:5000 detected RhoA family GTPase in 10ug of HL-60 cell lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice