IgM, Fc (APC)

Size

£499.00

SKU:: 052435
Application:: Flow Cytometry
Purity:: Purified by size-exclusion chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified human IgM.
Clone:: CH2
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Extra Details:: Immunoglobulin M (IgM) is produced as a 900kD pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12g/l and its biological survival (plasma T1/2) is 5 days. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1-4ug/ml. Extracellular and intracellular staining. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.