052427
MPC2 (Mitochondrial Pyruvate Carrier 2, Brain Protein 44, BRP44)
Size
£563.00
- SKU:
- 052427
- Purity:
- Purified by Protein G affinity chromatography from hybridoma tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Peptide coupled to KLH.
- Clone:
- JCM-1
- Format:
- Supplied as a liquid in PBS, 0.02% sodium azide, 25% glycerol.
- Extra Details:
- Pyruvate is the end-product of glycolysis, a major substrate for oxidative metabolism, and a branching point for glucose, lactate, fatty acid and amino acid synthesis. The mitochondrial enzymes that metabolize pyruvate are physically separated from cytosolic pyruvate pools and rely on a membrane transport system to shuttle pyruvate across the impermeable inner mitochondrial membrane (IMM). Two proteins, mitochondrial pyruvate carriers MPC-1 and MPC-2, form a heterooligomeric complex in the IMM to facilitate pyruvate transport. This step is required for mitochondrial pyruvate oxidation and carboxylation-critical reactions in intermediary metabolism that are dysregulated in several common metabolic diseases. Applications: Suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Immunoprecipitation: 1:200 Immunocytochemistry: 1:400 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice