Acid Phosphatase, Non-Prostatic, Sweet Potato (ACP)

Size

£646.00

SKU:: 052413
Molecular Weight:: 110000
Purity:: Chromatographically purified
Storage Conditions:: -20°C
Supplier:: United States Biological
CAS Number:: 9001-77-8
Format:: Supplied as a light purple lyophilized powder in 50mM citrate, 1g/L trehalose, 1g/L PEG 20,000, pH 6.5.
Extra Details:: Sweet potato acid phosphatase has been used in a study as a pre-column enzyme reactor via a covalent bond with glutaraldehyde and aminopropyl controlled-pore glass. It has also been used in a study to investigate the use of tyrosine, histidine, and cysteine as ligands for Mn(III) in sweet potato phosphatase. Source: Sweet Potato Purity: ≥300 U/mg protein Activity: ≥30 U/mg solid Unit Definition: One unit will catalyze the hydrolysis of one micromole of p-nitrophenylphosphate to p-nitrophenol and phosphate/minute at pH 4.8 and 37°C. Protein: Reported mg protein/mg solid (Coomassie) Contaminants: beta-Amylase: ≤50% alpha-Amylase: ≤1% Activity: As Reported Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.