Antibacterial Peptide LL-37, Citrullinated

Size

£767.00

SKU:: 052393
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Citrullinated human LL-37 peptide
Clone:: 6A8
Format:: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, 50% glycerol.
Extra Details:: LL-37 is a cationic α-helical peptide expressed in human bone marrow, testis, granulocytes, and gingival epithelium and a variety of immune cells. It has antimicrobial and antiviral activity, and protein levels of LL-37 are increased in epithelial cells, macrophages, and neutrophils following bacterial infection in vitro. LL-37 can be citrullinated by protein arginine deiminase 2 (PAD2) and PAD4, a modification that reduces its antibacterial and antiviral activities. Citrullinated, unlike native, LL-37 does not prevent mortality in a mouse model of D-galactosamine-sensitized endotoxic shock. Citrullinated LL-37 has been found in neutrophil extracellular traps (NETs), as well as the serum of patients with rheumatoid arthritis (RA) and individuals without RA. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1:500-1:1000 ELISA: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice