052392
Interleukin-6, Citrullinated
Size
£927.00
- SKU:
- 052392
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic citrullinated peptide corresponding to an internal region of human IL-6
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Interleukin-6 (IL-6) is a cytokine with roles in both the initiation and resolution of inflammatory responses. IL-6 is produced in response to an inflammatory event, induces recruitment of neutrophils to the site of inflammation, and signals through the membrane-bound IL-6 receptor (IL-6R) to upregulate the synthesis of acute phase proteins, lipolysis in the liver, and induced differentiation of T and B cells. Following initiation of this classical signaling pathway, a soluble form of IL-6R (sIL-6R) is released from neutrophils to promote recruitment of monocytes and macrophages, induction of fever via IL-6 action in the hypothalamus, and production of anti-inflammatory M2 macrophages to initiate tissue repair. Production of IL-6 is dysregulated in various chronic inflammatory diseases, including rheumatoid arthritis, Castleman disease, polymyalgia rheumatica, and giant cell arteritis. It also induces tumor growth and metastasis via activation of JAK/STAT signaling. Recombinant human IL-6 can be citrullinated at arginine residues by protein arginine deiminase 4 (PAD4) in vitro. Citrullinated IL-6 and anti-citrullinated peptide antibodies (ACPAs) reactive with citrullinated IL-6 have been found in synovial fluid and sera, respectively, from patients with rheumatoid arthritis. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1:100 ELISA: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice