052390
Myelin Basic Protein, Citrullinated
Size
£947.00
- SKU:
- 052390
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to the internal region of human myelin basic protein (isoform 5) with citrullines at R122 and R130
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Citrullinated myelin basic protein (citMBP) is a citrullinated form of MBP, a protein that is integral to myelin stability in the CNS. MBP contains 19 arginine residues that can be citrullinated by peptidylarginine deiminases (PADs), decreasing the positive charge of MBP. The loss of positive charge disrupts MBP-lipid interactions, leading to myelin destabilization and loss and increases its susceptibility for degradation. MBP is degraded by the myelin-associated protease cathepsin D, which leads to exposure and release of immunodominant epitopes. The percentage of MBP that is citrullinated is increased in the postmortem brain from patients with multiple sclerosis (MS), with an even greater percentage citrullinated in Marburg disease, a more severe form of MS. citMBP isolated from patients with multiple sclerosis (MS) is degraded by cathepsin D at a higher rate than MBP isolated from healthy controls. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1:200 ELISA: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice