Norovirus (MNV-1)

Size

£767.00

SKU:: 052319
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Infectious and UV-inactivated murine norovirus-1.
Clone:: 5C4.10
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Extra Details:: Norovirus is a member of the Caliciviridae family and is responsible for gastroenteritis outbreaks. Murine norovirus (MNV-1) that is about 28 to 35 nm in diameter, shares many biochemical and genetic features with human noroviruses. An analysis of the MNV-1 genome shows three open reading frames (ORF) that are characteristic of noroviruses and vesiviruses. ORF1 is reported to encode a predicted 187kD polyprotein that contains the 2C helicase, 3C protease, and 3D polymerase motifs. ORF2 encodes a 59kD capsid protein that can self-assemble into virus-like particles when expressed in a baculovirus expression system. ORF3 is reported to encode a putative 22kD basic protein. The norovirus capsid protein contains a highly conserved shell (S) and protruding (P) domain and the P domain is shown to contain binding sites for receptors and neutralizing antibodies. The S domain is shown to be highly conserved and the capsid sequence diversity is limited to the P domain. MNV-1 infection is reported to modulate the MAPK pathway to activate eIF4E phosphorylation and the activation of p38 and Mnk during MNV-1 infection is considered to be important for its replication. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/mL detected Norovirus (MNV-1) in RAW 264.7 cells infected with Murine norovirus-1.CW3. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice