Glucuronoxylomannan (GXM)

Size

£716.00

SKU:: 052311
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Cryptococcus neoformans serotype A glucuronoxylomannan (GXM)-Tetanus Toxoid conjugate vaccine.
Clone:: 18B7
Format:: Supplied as a liquid in PBS. No preservative added.
Extra Details:: Cryptococcus neoformans is an encapsulated yeast-like fungus that is reported to causes systemic infections, including fatal meningoencephalitis. Glucuronoxylomannan (GXM) is the principal constituent of the Cryptococcus neoformans capsule that modulates the inflammatory response of human monocytes in vitro. The basic structural unit of GXM is a tri-mannose repeat with a glucuronic acid residue in the first mannose. Its structure is shown to be further modified in individual strains by the addition of xylose substitutions on the mannan backbone. Six triads known as M1-M2 are shown to be present in various proportions in GXM from the various serotypes and M2 is reported to be the most common triad in serotype A GXM. Soluble GXM serves as an important virulence factor and contributes to the pathogenesis of infection, and high levels of GXM have been found in serum of patients with cryptococcosis. 052311 displays significant activity against GXM and is reported act by possibly hydrolyzing the oligosaccharide substrate. This antibody acts as a potent opsonin for Cryptococcus neoforman by increasing its phagocytosis. 052311 may also reduce GXM levels by interfering with the release of polysaccharide from the capsule. Administration of this antibody to mice is shown to rapidly clear serum Cryptococcal antigen. Applications: Suitable for use in ELISA, Immunodepletion, Immunocytochemistry, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunocytochemistry: 1:1000 detected Glucuronoxylomannan (GXM) in heat killed cryptococcus cells. Immunohistochemistry (paraffin): 1:50-1:250 detected Glucuronoxylomannan (GXM) in S. neoformans-infected mouse spleen, mouse lung, mouse liver, and mouse brain tissues. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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