052268-BIOTIN

IL17A (Interleukin-17A) (Biotin)

Size

£505.00

SKU:: 052268-BIOTIN
Purity:: Purified by size-exclusion chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Mammalian-derived human IL-17-IgG fusion protein, boost with recombinant human IL-17A
Clone:: 9F9
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Labeled with Biotin.
Extra Details:: Interleukin 17A (IL-17A) is a proinflammatory cytokine produced by activated T cells. IL-17A-mediated downstream pathways induce the production of inflammatory molecules, chemokines, antimicrobial peptides, and remodeling proteins. It plays an important role in connecting T cell-mediated adaptive immunity and acute inflammatory response to destroy extracellular bacteria and fungi. It is the signature effector cytokine of Th17 cells, and in this role it primarily induces neutrophil activation and recruitment at infection and inflammatory sites. High levels of IL-17A are associated with rheumatoid arthritis, psoriasis, multiple sclerosis, and another inflammatory diseases, including lung injugy during severe COVID 19. This cytokine also contributes to germinal center formation by regulating the chemotactic response of B cells to CXCL12 and CXCL13, enhancing retention of B cells within the germinal centers, B cell somatic hypermutation rate and selection toward plasma cells. It is an effector cytokine for invariant NKT cells (iNKT), and it is involved in epithelial barrier formation upon injury. Applications: Suitable for use in ELISA, Immunoprecipitation, Flow Cytometry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 0.5-4ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice