052254-PE
Arrestin-C (Cone Arrestin, Retinal Cone Arrestin, ARR3, Retinal Cone Arrestin-3) (PE)
Size
£1,023.00
- SKU:
- 052254-PE
- Purity:
- Purified by Protein G affinity chromatography
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Crude retinal extract from Monkey (Macaca fascicularis). Epitope: N-terminal
- Clone:
- 7G6
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- Arrestin-C (UniProt: A0A2K5U9A7; also known as Cone arrestin, Retinal cone arrestin-3) is encoded by the ARR3 gene (Gene ID: 102134564) in Monkey. Arrestins are a superfamily of multi-functional proteins that that regulate signaling and trafficking of the majority of G-protein-coupled receptors (GPCRs), as well as sub-cellular localization and activity of many other signaling proteins. Arrestin-C is predominantly found in inner and outer segments, and the inner plexiform regions of the retina. It is expressed in cone photoreceptors and pinealocytes and may contribute to the shut-off mechanisms associated with high acuity color vision. Arrestin-C is an elongated two-domain molecule with overall fold and key inter-domain interactions that hold the free protein in the basal conformation similar to the other subtypes. Several structural elements are reported to contribute to arrestin binding. The C-terminal acidic region serves a regulatory role in controlling arrestin binding selectivity toward the phosphorylated and activated form of a receptor. The basic N-terminal domain directly participates in receptor interaction and serves a regulatory role via intramolecular interaction with the C-terminal acidic region. Also, two centrally localized domains are directly involved in determining receptor binding specificity and selectivity. Clone 7G6 is a cone-specific marker that recognizes all cones in the adult primate retina. In fetal retina, although long and middle wavelength-sensitive cones recognized, a subset of short wavelength-sensitive cones are shown to be delayed in their reactivity to this clone. Applications: Suitable for use in Immunofluorescence, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice