052239
nNOS (Neuronal Nitric Oxide Synthase, NOS I)
Size
£700.00
- SKU:
- 052239
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to human nNOS at C-terminal.
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Nitric oxide synthase (NOS) catalyzes the oxidation of arginine to nitric oxide (NO) and citrulline. Three distinct isoforms of NOS have been described having nomenclature based on the tissue source from which they were originally cloned. These three isoforms are neuronal/brain NOS (nNOS/bNOS/NOS-I), inducible NOS (iNOS/NOS-II), and endothelial NOS (eNOS/NOS-III). nNOS is a soluble enzyme found in brain, the peripheral nervous system and skeletal muscle. An alternately spliced form of nNOS (nNOSμ) containing a 34 amino acid insert has been identified in skeletal muscle. In neurons, protein-protein interactions with PSD95 and PSD93 via the PZD domain at the N-terminus of nNOS localizes the enzyme with NMDA receptors. Although nNOS was originally thought to be constitutively expressed, abundant evidence suggests expression is regulated by a variety of conditions. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Formalin fixed paraffin embedded): 1:200 using heat induced antigen retrieval in citrate buffer, pH 6.0. Immunocytochemistry: 1:500 Western Blot: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice