AIDA1 (Ankyrin Repeat and Sterile alpha Motif Domain-containing Protein 1B, Amyloid-beta Protein Intracellular Domain-associated Protein 1, EB-1)

Size

£748.00

SKU:: 052227
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Linear peptide corresponding to 10 amino acids from the SAM1/2 domain in the C-terminal half of rat AIDA1/ANKS1B. Species Sequence Homology (100%): human, canine, feline, porcine and monkey
Clone:: 2K9
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Extra Details:: Ankyrin repeat and sterile alpha motif domain-containing protein 1B (UniProt: P0C6S7; also known as Amyloid-beta protein intracellular domain-associated protein 1, AIDA-1, E2A-PBX1-associated protein, EB-1) is encoded by the Anks1b gene (Gene ID: 314721) in rat. AIDA-1 is an adaptor protein that has been implicated in Cajal body and nucleolar function. It is expressed throughout the brain and is highly expressed in the hippocampus. It is one of the most abundant proteins at postsynaptic densities where it is reported to bind to PSD95 and associates with NMDA receptors (NMDARs). In the brain, it interacts with amyloid b precursor protein and plays a role in brain development. AIDA-1 preferentially associates with GluN2B-containing NMDARs and plays a role in their transport from the endoplasmic reticulum (ER). Loss of AIDA-1 results in the accumulation of GluN2B in the ER and decreases GluN2B and increases GluN2A distribution at the synapse. However, loss of AIDA-1 does not affect AMPAR-mediated synaptic transmission or the probability of glutamate release. AIDA-1 has seven Ankyrin repeats in its N-terminal half and has two closely juxtaposed sterile alpha motif (SAM) domains (aa 810-876 and 884-949). Within the second SAM domain resides the nuclear localization signal (aa 935-938). Mutations in Anks1b gene are known to affect GluN2B function and are linked to the pathogenesis of neuropsychiatric disorders. Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 detected AIDA1 in rat testis tissue lysates under non reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice