052221X
CD261 (TRAIL Receptor 1, TRAIL-R1, Death Receptor 4, DR4, APO2, TNFRSF10A) (Low Endotoxin/Azide Free)
Size
£512.00
- SKU:
- 052221X
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Fusion protein containing the extracellular part of TRAIL-R1 and the constant part of the heavy chain of the human IgG1.
- Clone:
- DR-4-02
- Format:
- Supplied as a liquid in PBS, pH 7.4. No preservative added.
- Extra Details:
- TRAIL-R1 (CD261, DR4) is a type I transmembrane protein, also called TRAIL receptor 1. The ligand for this DR4 death receptor has been identified and termed TRAIL, which is a member of the TNF family. DR4, as many other receptors (Fas, TNFR1, etc.), mediates apoptosis and NF kappaB activation in many cells and tissues. Apoptosis, a programmed cell death, is a operating process in normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by coupled of certain cytokines (TNF family - TNF, Fas ligand) and their death domain containing receptors (TNFR1, Fas receptor). Applications: Suitable for use in Flow Cytometry, Functional Assays, Immunocytochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Functional Application: 052221X blocks apoptosis triggered by a ligand (TRAIL). Plastic-immobilized (cross-linked) antibody induces apoptosis in sensitive cells. Recommended dilution of antibody: 2-3ug/ml in cultivation medium. The final concentration of TRAIL is 20-200ng/ml. Add the antibody 15 min before addition of TRAIL. Flow Cytometry: 2-4ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice