052211
Reticulon-4 (RTN, NOGO-A, Neurite Outgrowth Inhibitor)
Size
£774.00
- SKU:
- 052211
- Application:
- IHC-Fr
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Hamster
- Immunogen:
- Lateral olfactory tract of E14.5 mouse embryos fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS), washed and suspended in PBS.
- Clone:
- NG1
- Format:
- Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
- Extra Details:
- Reticulon-4 (UniProt: Q99P72; also known as Neurite outgrowth inhibitor, Nogo protein) is encoded by the Rtn4 (also known as Kiaa0886, Nogo) gene (Gene ID: 68585) in murine species. NOGO proteins are required for the formation and stabilization of endoplasmic reticulum (ER) tubules. They are also involved in nuclear envelope expansion, nuclear pore complex formation and proper localization of inner nuclear membrane proteins. Three isoforms of NOGO have been described that share a common C-terminal domain but differ in their N-terminal sequence. NOGO-A is the longest isoform and is considered as a developmental neurite growth factor. It acts as a negative regulator of axon-axon adhesion and growth, but also acts as a facilitator of neurite branching. In the adult central nervous system (CNS), it is involved in down-regulation of growth, stabilization of wiring, and restriction of plasticity. It can also serve as a negative regulator of CNS angiogenesis by blocking spreading, migration, and sprouting of primary brain microvascular endothelial cells. Isoform B mainly functions in the endothelial cells and vascular smooth muscle cells. It promotes the migration of endothelial cells but inhibits the migration of vascular smooth muscle cells. Isoform C regulates cardiomyocyte apoptosis under hypoxic conditions. This clone has been use to show that knockdown of NOGO-A reduces the number of axonal collateral branches in olfactory bulb. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (frozen): 1:200 detected RTN/NOGO-A in E14 mouse embryo frozen tissue sections. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice