NK1R (Neurokinin-1 Receptor, TACR1, Tachykinin Receptor-1)

Size

£825.00

SKU:: 052209
Application:: ICC
Purity:: Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: HEK293 human embryonic kidney cell line transfected with human NK1R
Clone:: 24K1
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: NK1R (Neurokinin-1 Receptor), gene name TACR1 (Tachykinin Receptor-1), is an ~80kD 7-transmembrane glycoprotein receptor for the proinflammatory tachykinin neuropeptide, Substance P. NK1R is constitutively or inducibly expressed on a wide variety of cells, including monocytes, macrophages, microglia, lymphocytes, neutrophils, mast cells, and neurons. The 407 amino acid (aa) human NK1R contains 89 extracellular aa over multiple segments which collectively share 92% aa identity with corresponding regions of mouse and rat NK1R. An ~50kD short isoform that ends at aa 311 lacks almost all of the C-terminal cytoplasmic signaling region. It is the only form expressed in human monocytes and undifferentiated THP-1 cells, and its function appears to differ from the long isoform. Applications: Suitable for use in Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunocytochemistry: 8-25ug/ml for immersion fixed THP-1 human acute monocytic leukemia cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder and reconstituted products must be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Reconstitute with sterile PBS. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice