052191
Interferon Regulatory Factor 3 (IRF3)
Size
£863.00
- SKU:
- 052191
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to aa206-427 from human IRF3, expressed in E. coli.
- Clone:
- 24J3
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Interferon regulatory factor 3 (IRF3) is a 50 kDa, 427aa, member of the IRF family of proteins. Human IRF3 is a transcription factor that binds the interferon-sensitive response element (ISRE) and is activated by Toll-like receptors, TLR3 and TLR4. Viral entry or engagement of TLR3 or TLR4 stimulates IRF3 phosphorylation, nuclear translocation and stimulation of IFN production Applications: Suitable for use in Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml in lysates of Raji human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, and Jurkat human acute T cell leukemia cell line Flow Cytometry: 2.5ug labels 10e6 cells in Daudi Human Cell Line Optimal dilutions to be determined by researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer,
- Shipping Conditions:
- Blue Ice