052185-BIOTIN
Polyethylene Glycol (PEG) (Biotin)
Size
£971.00
- SKU:
- 052185-BIOTIN
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- KLH-PEG with terminal methoxy group.
- Clone:
- RM105
- Format:
- Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide, 50% glycerol. Labeled with Biotin.
- Extra Details:
- Polyethylene glycols (PEGs) are synthetic and hydrophilic polymers. They are linear or branched and contain a reactive end group, such as acrylate, methacrylate, dibenzocyclooctynol, or vinyl sulfonate, for covalent attachment to macromolecules or linkers. The opposite end group of PEGs is commonly a methyl group (methoxy PEG), however, hydroxy, amino, butoxy, and tert-butoxy end groups have also been used. PEGs are non-toxic and are commonly used to prolong the in vivo circulation time of pharmaceutical agents. Free PEGs are non-immunogenic but become immunogenic when conjugated to a drug delivery nanosystem (DDS) or a macromolecule. Immunogenicity of PEGs varies based on polymer length and branching, end group composition, and chemical nature of the PEG acceptor structure. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry Other applications not tested. Recommended Dilutions: ELISA: 0.02-0.5ug/ml Immunohistochemistry (paraffin): 0.5-2ug/ml Western Blot: 0.1-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice