Platelet Derived Growth Factor Receptor Type B (PDGFRb, CD140b)

Size

£800.00

SKU:: 052183
Purity:: Purified by affinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: KLH-conjugated linear peptide corresponding to a cytoplasmic sequence C-terminal to the kinase domain of PDGFR. Species Sequence Homology: human, bovine and canine
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide
Extra Details:: Platelet-derived growth factor receptor beta (EC 2.7.10.1; UniProt P09619; also known as Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, CD140b, PDGF-R-beta, PDGFR-1, PDGFR-beta, Platelet-derived growth factor receptor 1) is encoded by the PDGFRB (also known as IBGC4, IMF1, PDGFR, PDGFR1) gene (Gene ID 5159) in human. PDGFR-1 is a tyrosine-protein kinase that acts as a cell-surface receptor for homodimeric PDGFB and PDGFD and for heterodimers formed by PDGFA and PDGFB. PDGF receptors consist of extracellular domains with five immunoglobulin (Ig) - like domains and intracellular parts with kinase domains, which contain characteristic inserts of about 100 amino acid residues without homology to kinases. Ligand binding occurs mainly to Ig-like domains 2 and 3, which causes dimerization of the receptor, which is further stabilized by direct receptor-receptor interactions involving Ig-like domain 4. In the absence of a bound ligand, receptor is present in an inactive conformation. However, upon ligand binding it undergoes dimerization and autophosphorylation on tyrosine residues. PDGFR-1m plays an essential role in the regulation of embryonic development, cell proliferation, survival, differentiation, chemotaxis, and migration. It is also considered to be essential for endothelial cell proliferation, migration and recruitment of pericytes and smooth muscle cells to form blood vessels. PDGFR-1 phosphorylates phospholipase C-gamma 1 (PLCG1), PIK3R1, PTPN11, RASA1/GAP, CBL, SHC1 and NCK1. Activation of PLCG1 leads to the production of diacylglycerol and IP3 that are involved in calcium mobilization and activation of PKC. Applications: Suitable for use in Immunocytochemistry, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 2ug/ml detected PDGFR in 10ug of BALB/3T3 clone A31 cell lysate. Immunoprecipitation: 5ug immunoprecipitated PDGFR from 500ug of BALB/3T3 clone A31 cell lysate. Immunocytochemistry: 1:100 detected PDGFR immunoreactivity in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized BALB/3T3 clone A31 cells. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice