Serotonin Receptor 2A (HTR2A)

Size

£761.00

SKU:: 052171
Purity:: Purified by Protein G affinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: GST-tagged recombinant protein fragment corresponding to the third cytoplasmic domain of human Serotonin receptor 2A/HTR2A.
Clone:: 9B11.1
Format:: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Extra Details:: 5-hydroxytryptamine receptor 2A (UniProt P28223; also known as 5-HT-2, 5-HT-2A, 5-HT2A, Serotonin receptor 2A) is encoded by the HTR2A (also known as HTR2) gene (Gene ID 3356) in human. Hydroxytryptamine (5-HT) is synthesized in enterochromaffin cells in the intestine and in serotonergic nerve terminals. In the periphery, 5-HT mediates gastrointestinal motility, platelet aggregation, and contraction of blood vessels. Many functions of the central nervous system are influenced by 5-HT, including sleep, motor activity, sensory perception, arousal and appetite. A family of 12 GPCRs and one ion channel mediate the biological effects of 5-HT. 5-HT2A receptor, which couples to Gq/11 to increase intracellular calcium, is widely expressed at central and peripheral sites of 5-HT action and contributes to many of the physiological effects of 5-HT. The hallucinogenic activity of LSD is mediated in part by its action as a partial to full agonist of 5-HT2A receptor, and the activity of atypical antipsychotics such as clozapine appears to be mediated in part by antagonism against 5-HT2A receptor. Human 5-HT2A receptor contains four extracellular domains (a.a. 1-75, 133-148, 216-233, 347-362), seven transmembrane segments (a.a. 76-99, 111-132, 149-171, 192-215, 234-254, 325-346, 363-384), and four cytoplasmic domains (a.a. 100-110, 172-191, 255-324, 385-471). Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry (FFPE): 1:50 detected 2A/HTR2A in human cerebral cortex, mouse cerebral cortex, and rat testis tissue sections. Requires HIER. Western Blot: 0.5ug/ml detected 2A/HTR2A in 10ug of human cerebral cortex tissue lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice