Serotonin Receptor 1B (5-hydroxytryptamine receptor 1B, 5-HT-1B, 5-HT-1D-beta, 5-HT1B)

Size

£761.00

SKU:: 052170
Purity:: Purified by Protein G affinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: GST-tagged recombinant protein fragment corresponding to the thrid cytoplasmic domain of human serotonin receptor 1B.
Clone:: 1C2.1
Format:: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Extra Details:: 5-hydroxytryptamine receptor 1B (UniProt P28222; also known as 5-HT-1B, 5-HT-1D-beta, 5-HT1B, S12, Serotonin 1D beta receptor, Serotonin receptor 1B) is encoded by the HTR1B (also known as HTR1DB) gene (Gene ID 3351) in human. The 5-HT receptors are grouped according to their antagonist susceptibilities and their affinities for 5-HT. The 5-HT1 receptors are a subfamily of 5-HT receptors that were originally classified according to their inhibition of adenylyl cyclase, degree of sequence similarity and their overlapping pharmacological specificities. The subfamily has five different receptors: 5-HT1A, 5-HT1B, 5-HT1D, 5-HT1E, 5-HT1F that can couple to Gi/Go and mediate inhibitory neurotransmission. 5HT-1B receptor belong to the family of G-protein coupled receptors that are distributed in brain cortex, striatum, amygdala, medulla, hippocampus, caudate nucleus and putamen. 5-HT-1B also functions as a receptor for ergot alkaloid derivatives, various anxiolytic and antidepressant drugs and other psychoactive substances, such as lysergic acid diethylamide. Ligand binding causes a conformation change in the receptor that triggers signaling via G-proteins, which modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling via 5-HT-1B inhibits adenylate cyclase activity. 5-HT1B receptor is known to be involved in thermoregulation, respiration, appetite control, sexual behavior, and aggression. 5-HT-1B receptor are therapeutic targets for antimigraine drugs and for treatment of bipolar disorders, autism, and anti-aggressive agents. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry (FFPE): 1:50-1:250 detected serotonin receptor 1B in human (cerebellum and cerebral cortex) and rat (cerebral cortex and medulla) brain tissue sections. Requires HIER. Western Blot: 1ug/ml detected serotonin receptor 1B in 10ug of mouse brain tissue lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice