CD252 (Tumor Necrosis Factor Ligand Superfamily Member 4, Glycoprotein Gp34, OX40 Ligand, OX40L, TAX Transcriptionally-activated Glycoprotein 1, TNFSF4, TXGP1)

Size

£889.00

SKU:: 052164
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant. Endotoxin: ≤0.1EU/ug (LAL).
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Gln51-Leu183 from human CD252, expressed in NS0 cells.
Clone:: 11n40
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: OX40 Ligand, also known as gp34, is a type II transmembrane glycoprotein belonging to the TNF superfamily and is now designated TNFSF4. Engagement of OX40 Ligand by OX40 (CD134) on activated CD4+ T cells promotes the proliferation and differentiation of activated B cells. Applications: Suitable for use in Flow Cytometry, Immunocytochemistry and Neutralization. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug/1x10e6 cells Immunocytochemistry: 8-25ug/ml for immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS. Neutralization: 0.1-0.6ug/ml in the presence of 10ng/ml recombinant human CD252. Measured by its’ ability to neutralize CD252 in the HT1080 human fibrosarcoma cell line transected with human CD252. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice