052158
KI67 (MKI67)
Size
£544.00
- SKU:
- 052158
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant construct containing the 2nd, 3rd and 4th Ki67 repeats of the human sequence (aa1111-1490), expressed in E. coli.
- Clone:
- 6B4
- Format:
- Supplied as a liquid in PBS, 0.03% sodium azide, 50% glycerol.
- Extra Details:
- The Ki67 proteins were first discovered in an attempt to generate cancer specific monoclonal antibodies. A monoclonal antibody which bound to structures in the nuclei of dividing but not quiescent cells was produced and shown to bind two very large proteins of molecular weight 345kD and 395kD. The two proteins were derived from alternate transcripts of a single gene. The presence of Ki67 proteins, detected with an appropriate antibody, is an indicator of cell proliferation and the level of Ki67 expression is one of the most reliable biomarkers of proliferative status of cancer cells. Recent studies show that Ki67 coats dividing chromosomes and ensures that each one enters the appropriate daughter cell. Applications: Suitable for use in Immunocytochemistry/Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 Immunocytochemistry/Immunofluorescence: 1:2000-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice