052138
Autotaxin (ENPP2, ATX, PDNP2)
Size
£825.00
- SKU:
- 052138
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- KLH-conjugated linear peptide corresponding to human Autotaxin at the substrate binding region. Species Sequence Homology: mouse, rat, canine and bovine
- Format:
- Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
- Extra Details:
- Autotaxin (also known as Enpp2), an exo-enzyme that is upregulated in various cancers such as breast and lung cancer, is a secreted protein integral to the production of the lipid signaling molecule, lysophosphatidic acid (LPA). Wutotaxin is synthesized as a pre-pro-enzyme and stimulates cell proliferation, cell motility, and angiogenesis. Studies have shown increased expression of autotaxin in tumors is associated with increased tumor aggressiveness. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.5ug/ml detected Autotaxin in 10ug of oligodendrocyte precursor cell lysate. Western Blot performed with Tween. An uncharacterized bands may appear at ~35kD and 50kD in some lysates. Immunohistochemistry (paraffin): 1:400 detected Autotaxin in human cerebral cortex and ductal carcinoma tissues. Heat-induced epitope retrieval in citrate buffer, pH 6.0 was used Optimal dilutions to be determined by the researcher. Control: Oligodendrocyte precursor cell lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice