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052132

GLUT2 (Glucose Transporter Type 2, Solute Carrier Family 2, Facilitated Glucose Transporter Member 2, SLC2A2)

Size

£1,010.00

SKU:
052132
Purity:
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Rat
Immunogen:
CHO cell line transfected with mouse Glut2. Epitope: Met1-Val523
Clone:
24I7
Format:
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 400ul sterile PBS.
Extra Details:
Glut2 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains. Glut2 is expressed predominantly in liver, intestine, kidney and pancreatic beta-cells. It is a low-affinity glucose transporter that has been suggested to function as a glucose sensor in pancreatic beta-cells and facilitate either glucose uptake or efflux from cells depending on the nutritional state. Applications: Suitable for use in Flow Cytometry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunocytochemistry: 8-25ug/ml using immersion fixed beta TC-6 mouse beta cell insulinoma cell line Flow Cytometry: 2.5ug labels 10e6 cells using beta TC-6 mouse beta cell insulinoma cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Conditions:
Blue Ice