eNOS (Endothelial Nitric Oxide Synthase, Endothelial NOS, cNOS) (Not for Export EU)

Size

£698.00

SKU:: 052120
Application:: WB
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide from the C-terminal region of human eNOS
Format:: Supplied as a lyophilized powder. Reconstitute with 100ul sterile ddH2O.
Extra Details:: eNOS catalyzes the formation of NO from L-arginine in many cell types including vascular endothelium, bronchiolar epithelium, cardiac myocytes, spleen, and kidney. Although eNOS was initially characterized as a constitutive enzyme, expression of eNOS mRNA is altered by many factors including shear stress, fibroblast growth factor, hypoxia, and LPS. eNOS is myristoylated and palmitoylated at the N-terminus, modifications which are required for localization to the plasmalalemmal caveolae of endothelial cells. Additionally, eNOS is phosphorylated on Ser1179 by protein kinase Akt resulting in an increase in NOS activity. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.