Complement Component 3c (C3c), Human

Size

£384.00

SKU:: 052114
Molecular Weight:: 138000
Purity:: ≥95% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
CAS Number:: 80295-41-6
Format:: Supplied as a liquid in PBS, pH 7.4.
Extra Details:: Complement C3 is synthesized as a single chain of 185kD and is processed in the plasma to a disulfide-linked heterodimer consisting of an α-chain (115kD) and a β-chain (70kD). During complement activation, C3 is cleaved by C3 convertases to C3a (9kD) and C3b (176kD) comprised of the large C-terminal portion of the α-chain and the β-chain. Factor I further cleaves C3b into C3c (140kD), C3d (35kD), and C3g (7kD). The current protocol yields C3c, however intact C3 and/or C3a/C3b may be co-purified. It is thought that the complement system might play a role in many diseases with an immune component, such as Alzheimer's disease, asthma, lupus erythematosus, various forms of arthritis, autoimmune heart disease and multiple sclerosis. Deficiencies of the terminal pathway predispose to both autoimmune disease and infections (particularly meningitis). Protein: 0.5-5mg/ml (A280 E0.1% = 1.03) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.