052090
Myosin 7B/MHC14 (Hinge Region)
Size
£535.00
- SKU:
- 052090
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to amino acid residues in the hinge region from mouse MYH7B/MHC14, conjugated to KLH. This sequence is highly conserved in rat and human MYH7B, and has less than 50% identity with other MYH family members.
- Format:
- Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide, 50% glycerol.
- Extra Details:
- Myosin is a highly conserved, ubiquitous protein found in all eukaryotic cells, where it provides the motor function for diverse movements such as cytokinesis, phagocytosis, and muscle contraction. All myosins contain an amino-terminal motor/head domain and a carboxy-terminal tail domain. The class II myosins, consist of the conventional two-headed myosins that form filaments and are composed of two myosin heavy chain (MYH) subunits and four myosin light chain subunits. There are 15 MYH genes identified as Class II myosins and these include six skeletal muscle MYHs (MYH1, MYH2, MYH3, MYH4, MYH8, MYH13), three cardiac MYHs (MYH6, MYH7, MYH7B) and two non-muscle MYHs (MYH9, MYH10), as well as a smooth muscle MYH (MYH11). Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, detects a 215kD protein corresponding to the apparent molecular mass of MYH7B on SDS-PAGE immunoblots of mouse extraocular muscle and brain. Immunocytochemistry: 1:300 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice